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monoclonal antibody rc2  (Developmental Studies Hybridoma Bank)


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    Structured Review

    Developmental Studies Hybridoma Bank monoclonal antibody rc2
    Monoclonal Antibody Rc2, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 94/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/monoclonal+antibody+rc2/anti-Nestin%2Fradial+glial+marker/pm38095299-202-37-41
    Average 94 stars, based on 15 article reviews
    monoclonal antibody rc2 - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Bioprocessing:

    Article Title: Disruption of the Neurogenic Niche in the Subventricular Zone of Postnatal Hydrocephalic hyh Mice
    Article Snippet: The monoclonal antibodies against NCAM and PSA- NCAM, developed by J. M. Hessel and J. Dodd, the monoclonal antibody RC2, developed by M. Yamamoto, and the monoclonal antibody against BrdU, developed by S. F. Kaufman, were obtained from the Developmental Studies Hybridoma Bank that was developed under the auspices of the NICHD and maintained by the Department of Biological Sciences, The University of Iowa, Iowa City, IA.

    Article Title: Disruption of the Neurogenic Niche in the Subventricular Zone of Postnatal Hydrocephalic hyh Mice
    Article Snippet: Neural stem cells persist after embryonic development in the subventricular zone (SVZ) niche and produce new neural cells during postnatal life; ependymal cells are a key component associated with this neurogenic niche.. In the animal model of human hydrocephalus, the hyh mouse, the ependyma of the lateral ventricles is progressively lost during late embryonic and early postnatal life and disappears from most of the ventricular surface throughout its life span.. To determine the potential consequences of this loss on the SVZ, we characterized the abnormalities in this neurogenic niche in hyh mice.

    other:

    Article Title: Chondroitin sulfate glycosaminoglycans control proliferation, radial glia cell differentiation and neurogenesis in neural stem/progenitor cells.
    Article Snippet: Monoclonal antibody RC2 developed by M. Yamamoto was obtained from the Developmental Studies Hybridoma Bank developed under the auspices of the NICHD and maintained by The University of Iowa, Department of Biological Sciences, Iowa City, IA 52242.

    Incubation:

    Article Title: CXCL12 promotes the crossing of retinal ganglion cell axons at the optic chiasm.
    Article Snippet: .. The tissue was washed with PBS, blocked with PBS containing 10% goat serum and 0.2% Triton X-100 and incubated at 4 ̊C overnight for cryosections or 2 days for retinas with the following antibodies in blocking solution: monoclonal antibody RC2 (1:5; Developmental Studies Hybridoma Bank AB_531887) or mouse anti-BRN3A (1:100; Merck Life Sciences MAB1585) in combination with rabbit anti-mouse ZIC2 (Murillo et al., 2015) or rabbit anti-phosphohistone-H3 (1:100; Merck Life Sciences 06-570). .. The tissue was washed with PBS and incubated in the appropriate secondary antibody: Cy3-conjugated goat anti-mouse IgM (Jackson ImmunoResearch; 1:1500; RC2), Alexa Fluor-488 goat antimouse IgG (ThermoFisher Scientific; 1:500; BRN3A) or Cy3-conjugated goat anti-rabbit IgG (1:1500; Jackson ImmunoResearch; ZIC2 or phosphohistone-H3).

    Article Title: CXCL12 promotes the crossing of retinal ganglion cell axons at the optic chiasm
    Article Snippet: .. The tissue was washed with PBS, blocked with PBS containing 10% goat serum and 0.2% Triton X-100 and incubated at 4°C overnight for cryosections or 2 days for retinas with the following antibodies in blocking solution: monoclonal antibody RC2 (1:5; Developmental Studies Hybridoma Bank, AB_531887) or mouse anti-BRN3A (1:100; Merck Life Sciences, MAB1585) in combination with rabbit anti-mouse ZIC2 ( ) or rabbit anti-phosphohistone-H3 (1:100; Merck Life Sciences, 06-570). .. The tissue was washed with PBS and incubated in the appropriate secondary antibody: Cy3-conjugated goat anti-mouse IgM (1:1500; Jackson ImmunoResearch, RC2), Alexa Fluor-488 goat anti-mouse IgG (1:500; Thermo Fisher Scientific, BRN3A) or Cy3-conjugated goat anti-rabbit IgG (1:1500; Jackson ImmunoResearch, ZIC2 or phosphohistone-H3).

    Blocking Assay:

    Article Title: CXCL12 promotes the crossing of retinal ganglion cell axons at the optic chiasm.
    Article Snippet: .. The tissue was washed with PBS, blocked with PBS containing 10% goat serum and 0.2% Triton X-100 and incubated at 4 ̊C overnight for cryosections or 2 days for retinas with the following antibodies in blocking solution: monoclonal antibody RC2 (1:5; Developmental Studies Hybridoma Bank AB_531887) or mouse anti-BRN3A (1:100; Merck Life Sciences MAB1585) in combination with rabbit anti-mouse ZIC2 (Murillo et al., 2015) or rabbit anti-phosphohistone-H3 (1:100; Merck Life Sciences 06-570). .. The tissue was washed with PBS and incubated in the appropriate secondary antibody: Cy3-conjugated goat anti-mouse IgM (Jackson ImmunoResearch; 1:1500; RC2), Alexa Fluor-488 goat antimouse IgG (ThermoFisher Scientific; 1:500; BRN3A) or Cy3-conjugated goat anti-rabbit IgG (1:1500; Jackson ImmunoResearch; ZIC2 or phosphohistone-H3).

    Article Title: CXCL12 promotes the crossing of retinal ganglion cell axons at the optic chiasm
    Article Snippet: .. The tissue was washed with PBS, blocked with PBS containing 10% goat serum and 0.2% Triton X-100 and incubated at 4°C overnight for cryosections or 2 days for retinas with the following antibodies in blocking solution: monoclonal antibody RC2 (1:5; Developmental Studies Hybridoma Bank, AB_531887) or mouse anti-BRN3A (1:100; Merck Life Sciences, MAB1585) in combination with rabbit anti-mouse ZIC2 ( ) or rabbit anti-phosphohistone-H3 (1:100; Merck Life Sciences, 06-570). .. The tissue was washed with PBS and incubated in the appropriate secondary antibody: Cy3-conjugated goat anti-mouse IgM (1:1500; Jackson ImmunoResearch, RC2), Alexa Fluor-488 goat anti-mouse IgG (1:500; Thermo Fisher Scientific, BRN3A) or Cy3-conjugated goat anti-rabbit IgG (1:1500; Jackson ImmunoResearch, ZIC2 or phosphohistone-H3).



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    Developmental Studies Hybridoma Bank monoclonal antibody rc2
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    Millipore mouse monoclonal antibody rc2
    Perturbation of vascular and neuronal development in E15 VegfΔTie2-Cre telencephalon. A–F, Reduced PECAM-1+ endothelial cells (B, yellow asterisk), laminin+ vessels (D, red arrows), and isolectin B4+ vessels (F, yellow arrows) in E15 VegfΔTie2-Cre pallium (B, D, F) compared with Vegffl/fl pallium (A, C, E). C, E, Orange arrows indicate well-formed vascular network in Vegffl/fl pallium. G, H, Ki67 labeling marks the proliferating progenitor pool in Vegffl/fl telencephalon (G), whereas Ki67-positive progenitors spanned the entire pallium of VegfΔTie2-Cre embryos (H). Boxed regions were magnified; white asterisks indicate Ki67-negative CP (G), and yellow asterisks indicate abnormal proliferation (H). I, J, MAP2 immunoreactivity reveals normal neuronal differentiation zone of E15 Vegffl/fl pallium (I, white asterisks), which is reduced in VegfΔTie2-Cre pallium (J, yellow asterisks). A band of MAP2+ cells were abnormally positioned in the VegfΔTie2-Cre IZ-SVZ (J, yellow arrows). K, High-magnification image of MAP2+ neurons in the VegfΔTie2-Cre SVZ. L, M, Tbr1 immunoreactivity in E15 Vegffl/fl (L) and VegfΔTie2-Cre (M) pallium. Boxed regions are magnified. The uniform arrangement of CP cells in Vegffl/fl pallium (L, white asterisk) was altered in VegfΔTie2-Cre pallium with abnormally thicker (M, yellow arrow) and thinner (M, yellow asterisk) cell arrangement in the CP. N, O, <t>RC2</t> immunostaining reveals continuous radial glial fibers extending from VZ to pial surface in Vegffl/fl pallium (N) and shortened radial glial processes in VegfΔTie2-Cre pallium (O, red asterisks). P, Q, GAD65/67 immunoreactivity shows decreased stream of GABA neurons in VegfΔTie2-Cre pallium extending to the medial edge (Q, yellow asterisks) compared with Vegffl/fl pallium (P, white asterisks). R–U, Calbindin+ and calretinin+ neurons were decreased (S, U, yellow asterisks) in E15 VegfΔTie2-Cre pallium compared with Vegffl/fl pallium (R, T, white asterisks). Calretinin immunoreactivity was decreased in the hippocampal primodium of VegfΔTie2-Cre embryos (U, yellow arrow). V, W, Uniform calretinin labeling in MZ and SP of Vegffl/fl embryos (V) and irregular calretinin labeling outside MZ in VegfΔTie2-Cre telencephalon (W). W, The boxed region was magnified, and yellow arrows point to calretinin+ cells outside MZ. X, Y, CHL1 immunostaining reveals axonal tracts in Vegffl/fl (X) and VegfΔTie2-Cre (Y) pallium. Y, Yellow arrow points to displaced axonal tract. DAPI (blue) was used to label nuclei (A, B, G–M, P–Y). Scale bars: A (applies also to B, E–J, L, M, P–U, X, Y), 100 μm; C (applies also to D), 75 μm; N (applies to O, V, W), 50 μm; magnified inset in G (applies to magnified insets in H, L, M), 50 μm; W (magnified inset), 25 μm. n = 8.
    Mouse Monoclonal Antibody Rc2, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/monoclonal+antibody+rc2/mouse+monoclonal+antibody+v9/pmc03771035-36-8-15
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    Image Search Results


    Perturbation of vascular and neuronal development in E15 VegfΔTie2-Cre telencephalon. A–F, Reduced PECAM-1+ endothelial cells (B, yellow asterisk), laminin+ vessels (D, red arrows), and isolectin B4+ vessels (F, yellow arrows) in E15 VegfΔTie2-Cre pallium (B, D, F) compared with Vegffl/fl pallium (A, C, E). C, E, Orange arrows indicate well-formed vascular network in Vegffl/fl pallium. G, H, Ki67 labeling marks the proliferating progenitor pool in Vegffl/fl telencephalon (G), whereas Ki67-positive progenitors spanned the entire pallium of VegfΔTie2-Cre embryos (H). Boxed regions were magnified; white asterisks indicate Ki67-negative CP (G), and yellow asterisks indicate abnormal proliferation (H). I, J, MAP2 immunoreactivity reveals normal neuronal differentiation zone of E15 Vegffl/fl pallium (I, white asterisks), which is reduced in VegfΔTie2-Cre pallium (J, yellow asterisks). A band of MAP2+ cells were abnormally positioned in the VegfΔTie2-Cre IZ-SVZ (J, yellow arrows). K, High-magnification image of MAP2+ neurons in the VegfΔTie2-Cre SVZ. L, M, Tbr1 immunoreactivity in E15 Vegffl/fl (L) and VegfΔTie2-Cre (M) pallium. Boxed regions are magnified. The uniform arrangement of CP cells in Vegffl/fl pallium (L, white asterisk) was altered in VegfΔTie2-Cre pallium with abnormally thicker (M, yellow arrow) and thinner (M, yellow asterisk) cell arrangement in the CP. N, O, RC2 immunostaining reveals continuous radial glial fibers extending from VZ to pial surface in Vegffl/fl pallium (N) and shortened radial glial processes in VegfΔTie2-Cre pallium (O, red asterisks). P, Q, GAD65/67 immunoreactivity shows decreased stream of GABA neurons in VegfΔTie2-Cre pallium extending to the medial edge (Q, yellow asterisks) compared with Vegffl/fl pallium (P, white asterisks). R–U, Calbindin+ and calretinin+ neurons were decreased (S, U, yellow asterisks) in E15 VegfΔTie2-Cre pallium compared with Vegffl/fl pallium (R, T, white asterisks). Calretinin immunoreactivity was decreased in the hippocampal primodium of VegfΔTie2-Cre embryos (U, yellow arrow). V, W, Uniform calretinin labeling in MZ and SP of Vegffl/fl embryos (V) and irregular calretinin labeling outside MZ in VegfΔTie2-Cre telencephalon (W). W, The boxed region was magnified, and yellow arrows point to calretinin+ cells outside MZ. X, Y, CHL1 immunostaining reveals axonal tracts in Vegffl/fl (X) and VegfΔTie2-Cre (Y) pallium. Y, Yellow arrow points to displaced axonal tract. DAPI (blue) was used to label nuclei (A, B, G–M, P–Y). Scale bars: A (applies also to B, E–J, L, M, P–U, X, Y), 100 μm; C (applies also to D), 75 μm; N (applies to O, V, W), 50 μm; magnified inset in G (applies to magnified insets in H, L, M), 50 μm; W (magnified inset), 25 μm. n = 8.

    Journal: The Journal of Neuroscience

    Article Title: Endothelial VEGF Sculpts Cortical Cytoarchitecture

    doi: 10.1523/JNEUROSCI.1368-13.2013

    Figure Lengend Snippet: Perturbation of vascular and neuronal development in E15 VegfΔTie2-Cre telencephalon. A–F, Reduced PECAM-1+ endothelial cells (B, yellow asterisk), laminin+ vessels (D, red arrows), and isolectin B4+ vessels (F, yellow arrows) in E15 VegfΔTie2-Cre pallium (B, D, F) compared with Vegffl/fl pallium (A, C, E). C, E, Orange arrows indicate well-formed vascular network in Vegffl/fl pallium. G, H, Ki67 labeling marks the proliferating progenitor pool in Vegffl/fl telencephalon (G), whereas Ki67-positive progenitors spanned the entire pallium of VegfΔTie2-Cre embryos (H). Boxed regions were magnified; white asterisks indicate Ki67-negative CP (G), and yellow asterisks indicate abnormal proliferation (H). I, J, MAP2 immunoreactivity reveals normal neuronal differentiation zone of E15 Vegffl/fl pallium (I, white asterisks), which is reduced in VegfΔTie2-Cre pallium (J, yellow asterisks). A band of MAP2+ cells were abnormally positioned in the VegfΔTie2-Cre IZ-SVZ (J, yellow arrows). K, High-magnification image of MAP2+ neurons in the VegfΔTie2-Cre SVZ. L, M, Tbr1 immunoreactivity in E15 Vegffl/fl (L) and VegfΔTie2-Cre (M) pallium. Boxed regions are magnified. The uniform arrangement of CP cells in Vegffl/fl pallium (L, white asterisk) was altered in VegfΔTie2-Cre pallium with abnormally thicker (M, yellow arrow) and thinner (M, yellow asterisk) cell arrangement in the CP. N, O, RC2 immunostaining reveals continuous radial glial fibers extending from VZ to pial surface in Vegffl/fl pallium (N) and shortened radial glial processes in VegfΔTie2-Cre pallium (O, red asterisks). P, Q, GAD65/67 immunoreactivity shows decreased stream of GABA neurons in VegfΔTie2-Cre pallium extending to the medial edge (Q, yellow asterisks) compared with Vegffl/fl pallium (P, white asterisks). R–U, Calbindin+ and calretinin+ neurons were decreased (S, U, yellow asterisks) in E15 VegfΔTie2-Cre pallium compared with Vegffl/fl pallium (R, T, white asterisks). Calretinin immunoreactivity was decreased in the hippocampal primodium of VegfΔTie2-Cre embryos (U, yellow arrow). V, W, Uniform calretinin labeling in MZ and SP of Vegffl/fl embryos (V) and irregular calretinin labeling outside MZ in VegfΔTie2-Cre telencephalon (W). W, The boxed region was magnified, and yellow arrows point to calretinin+ cells outside MZ. X, Y, CHL1 immunostaining reveals axonal tracts in Vegffl/fl (X) and VegfΔTie2-Cre (Y) pallium. Y, Yellow arrow points to displaced axonal tract. DAPI (blue) was used to label nuclei (A, B, G–M, P–Y). Scale bars: A (applies also to B, E–J, L, M, P–U, X, Y), 100 μm; C (applies also to D), 75 μm; N (applies to O, V, W), 50 μm; magnified inset in G (applies to magnified insets in H, L, M), 50 μm; W (magnified inset), 25 μm. n = 8.

    Article Snippet: Primary antibodies used for immunohistochemistry were as follows: mouse monoclonal antibody to RC2 (1:5, MAB5740, Millipore), goat polyclonal antibody to CHL1 (1:50, AF2147, R&D Systems), rat monoclonal antibody to CD31/PECAM-1 (1:50, 550274, BD Biosciences PharMingen), and rabbit polyclonal antibodies to laminin (1:10, L9393, Sigma), Ki67 (1:30, HPA000451, Sigma), microtubule associated protein 2 (MAP2; 1:50, M3696, Sigma), Tbr1 (1:25, ab31940, Abcam), GAD65/67 (1:50, AB1511, Millipore), calbindin (1:30, AB1778, Millipore), calretinin (1:30, 7699/3H, Swant), followed by secondary detection with AlexaFluor conjugates (Invitrogen).

    Techniques: Labeling, Immunostaining